| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Effects of growth phase and nitrogen limitation on biochemical composition of two strains of Tisochrysis lutea
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| Autores: |
Da Costa, Fiz
Le Grand, Fabienne
Quere, Claudie
Bougaran, Gael
Cadoret, Jean-paul
Robert, Rene
Soudant, Philippe
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| Data: |
2017-11
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| Ano: |
2017
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| Palavras-chave: |
Tisochrysis lutea
Nitrogen limitation
Growth phase
Flow cytometry
Fatty acids
Lipid classes
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| Resumo: |
Standard and mutant Tisochrysis lutea strains were grown in batch culture for 10 days under nitrogen (N)-replete and N-reduced conditions to determine the effects of N supply in culture medium and growth phase on microalgal physiology and biochemistry. These two T. lutea strains were compared in terms of growth, morphology, associated free-living bacterial community, viability, intracellular lipid content (as measured by Bodipy staining), chlorophyll autofluorescence, and biochemical composition, with a focus on lipid class and fatty acid compositions. The standard strain (T) reached higher cell counts regardless of N supply in culture medium. In both T. lutea strains, microalgal final cell density was significantly lower in N-reduced medium. Carbohydrates were enhanced at stationary phase in both strains, regardless of N supply. The oleaginous strain (T +) accumulated triacylglycerols; whereas, the standard strain T accumulated alkenones as reserve lipids, especially in N-reduced medium and stationary phase. Each T. lutea strain exhibited an intrinsic specific FA profile in neutral lipids (NL) and, to a lesser extent, in polar lipids (PL) independent of N supply and growth phase. T + contained more saturated and monounsaturated fatty acids (especially 14:0 and 18:1n-9), but less polyunsaturated fatty acids (18:4n-3 and 22:6n-3) than T. Overall, growth phase induced more changes in fatty acid profiles of both T. lutea strains in NL and PL than N supply in culture medium.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00399/51074/51936.pdf
DOI:10.1016/j.algal.2017.09.003
http://archimer.ifremer.fr/doc/00399/51074/
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| Editor: |
Elsevier Science Bv
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| Formato: |
application/pdf
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| Fonte: |
Algal Research-biomass Biofuels And Bioproducts (2211-9264) (Elsevier Science Bv), 2017-11 , Vol. 27 , P. 177-189
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| Direitos: |
2017 Elsevier B.V. All rights reserved.
info:eu-repo/semantics/openAccess
restricted use
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